GC-MS Determination of Oleic Acid in Tobacco Flavor with On-Line Pyrolytic Methylation

A method of GC-MS for the determination of oleic acid in tobacco flavor with on-line pyrolytic methylation was proposed. The sample of tobacco flavor was injected together with TMAH solution(derivation agent),and immediately methyl oleate was produced in gas injector port of 280℃. The DB-5MS capillary chromatographic column was used for GC separation and the selected ion monitoring mode was adopted in MS determination. Naphthalene was used as internal standard. Linearity range of oleic acid was found between 25 mg·kg^-1 and 1 000 mg·kg^-1, with detection limit (3S/N) of 0.35 mg·kg^-1 and lower limit of determination (10S/N) of 1.10 mg·kg^-1. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 92.6%-98.8%, with RSD's (n=6) in the range of 3.2%-6.3%.