HPLC Determination of 3 Kinds of Short Chain 4-(1,1,3,3-Tetramethylbutyl)phenol, Ethoxylated in Textiles

The sample was ultrasonically extracted twice with methanol. The extract was concentrated and then diluted with isopropanol. The analytes were seperated on a Dikama Platisil silica column (250 mm×4.6 mm, 5.0 μm), with N-hexane-isopropanol (85+15) as mobile phase A, isopropanol-water (85+15) as mobile phase B for gradient elution. Fluoresence detector was used with the excitation wavelength of 230 nm and the emission wavelength of 296 nm. Linearity ranges of the 3 kinds of 4-(1,1,3,3-tetramethylbutyl)phenol, ethoxylated were all 0.50-50 mg·L^-1, with detection limits (3S/N) of 0.30-0.40 mg·kg^-1. Recovery rates obtained by standard addition method were in the range of 94.4%-106% and RSDs (n=6) were less than 6.0%.