GC-MS/MS Analysis of Chemical Constituents of Essential Oil from Tobacco with Supercritical Fluid Extraction

Using tobacco as raw material, response surface methodology (RSM) was applied to optimization of supercritical-CO₂ fluid extraction (SCFE) of essential oil from tobacco, and chemical constituents of the essential oil were analyzed by gas chromatography-tandem mass spectrometry (GC-MS/MS). As shown by the results, the optimized extraction conditions were 20 422 kPa of extraction pressure, 38 ℃ of extraction temperature and 56 min of extraction time, and the yield of essential oil found was 0.73% under the extraction conditions. A total of 60 chemical constituents were separated and identified from the essential oil by SCAN model in GC-MS/MS, and the value of total peak areas of 60 chemical constituents was more than 90% of the total chromatographic peak area by MRM model in GC-MS/MS. RSDs (n=10) of retention time of each chemical composition were all less than 1.5%, and RSDs of the relative peak areas were all less than 10.0%.