HPLC-MS/MS Simultaneous Determination of 10 Diet Drugs in Health-Care Foods of Different Matrices with Solid Phase Extraction

HPLC-MS/MS was applied to the simultaneous determination of 10 diet drugs in health-care foods of different matrices with solid phase extraction. The health-care food sample was extracted ultrasonically with methanol, and the extract was purified by Oasis HLB solid phase extraction column. Zorbax-SB-C₁₈ chromatographic column was used as stationary phase, and the mixtures of 10 mmol·L^-1 ammonium acetate-0.1% (φ) formic acid solution and acetonitrile mixed in different ratios were used as mobile phase in gradient elution. ESI⁺ and multi-reactions monitor mode were adopted in MS/MS. Linear relationships between values of peak area and mass concentration of the 10 diet drugs were kept in the same range of 1.0-100.0 μg·L^-1, with detection limits (3S/N) in the range of 1.2-3.8 μg·kg^-1. On the base of blank sample, test for recovery was made by standard addition method. Values of recovery found were in the range of 79.5%-106%, with RSD's (n=6) in the range of 2.5%-9.7%.