HPLC-MS/MS Determination of Taurine in Health-Care Food

HPLC-MS/MS was applied to the determination of taurine in health-care food. An appropriate amount of the health-care food sample was weighed (to the nearest 0.001 g) and dissolved. The solution was diluted to a definite volume with water. Liquid sample could diluted with water directly. The sample solution obtained was used for analysis. BEH Amide chromatographic column was used as stationary phase, and mixtures of acetonitrile (A) and water (B) in different ratios were used as mobile phase in gradient elution. Mode of ESI^- and multi-reaction monitoring mode were adopted in MS/MS. Linear relationship between values of peak area and mass concentration of taurine was kept in the range of 0.01-2.0 mg·L^-1, with lower limit of determination (10S/N) of 1.0 mg·kg^-1. The proposed method was applied to the analysis of health food samples, giving values of recovery and RSD's (n=6) in the ranges of 94.0%-102% and 0.67%-3.9% respectively.