Determination of Dog-Originated Ingredient in Meat Products by Real-Time PCR Technology

According to dog's mitochondrial DNA (mtDNA) sequence, species-specific primer and probe were designed and real-time fluorescence PCR technology was applied to the determination of dog-originated ingredient in meat products. S-shaped amplification curves for the DNA samples collected from blood of 17 species of dogs were obtained while no such curve was obtained for either of the DNA samples collected from 14 other animal-originated meat; and RSD (n=7) of the values of Ct found from a same mother plate was 1.2%, which were taken as proof for good specificity and repeatability of the designed conditions of determination. Linear relationship between values of Ct and mass fractions of DNA of dog-originated ingredient was obtained in the range of 0.000 01% to 10%, with its sensitivity of 0.1 pg. No significant difference was observed among the results of various methods of processing of the meat products.