Determination of Sulfaguanidine by Flow Injection Chemiluminescence Based on Molecularly Imprinted Polymer

A molecularly imprinted polymer for specific recognition of sulfaguanidine was synthesized by using sulfaguanidine as the template molecule, diethylenetriamine as the monomer, and epoxy resin as the cross-linking reagent. Then it was used to prepare a chemiluminescent sensor for detemiantion of sulfaguanidine by flow injection chemiluminescence. After the sample solution was adsorbed by the sensor for 120 s, the sensor was washed with water for 30 s. Then sulfadiazine adsorbed on the sensor reacted with a mixed solution of 0.6 mol·L^-1 HCl-1×10^-3mol·L^-1 KMnO₄-8% (volume fraction) formaldehyde for 160 s to generate chemiluminescent signal. Linear relationship was found between chemiluminescence intensity with concentration of sulfaguanidine in the range of 5×10^-5-1.0×10^-3mol·L^-1, with detection limit (3S/N) of 1×10^-6mol·L^-1. Tests for recovery to compound sulfanilamide tablets were made by standard addition method, giving results in the range of 94.3%-98.5%, and RSDs (n=6) were less than 4.0%.