Determination of 8-Hydroxy-2'-deoxyguanosine Using Glassy Carbon Electrode Modified with Metal Organic Framework and Ordered Mesoporous Carbon

A metal organic framework material (ZIF-8) was prepared by a hydrothermal method. The glassy carbon electrode (GCE) was modified by ZIF-8 and ordered mesoporous carbon (OMC). Electrochemical behavior of the modified electrode (ZIF-8/OMC/GCE) was studied by cyclic voltammetry and alternating current impedance, and as shown by the results, the effective surface area of the modified electrode was increased significantly as compared with the bare GCE, thus leading to improvement of electrocatalytic activity of the modified electrode. Electrochemical characteristics of 8-hydroxy-2'-deoxyguanosine (8-OHdG) at ZIF-8/OMC/GCE were studied by differential pulse voltammetry, and it was found that linear relationship was obtained between values of current response and concentration of 8-OHdG in the range of 0.35-350 μmol·L^-1, with the detection limit (3S/N) of 0.22 μmol·L^-1. The modified electrode was used to determine 8-OHdG in human urine samples, giving values of recovery obtained by standard addition method in the range of 90.1%-108%.