Determination of Hydrogen Sulfide in Deteriorated Extracts for Tobacco by Ion Chromatography

A method of ion chromatography was proposed for determination of hydrogen sulfide in deteriorated extracts for tobacco. Under the conditions of acidification, H₂S was separated by water vapor distillation from deteriorated extracts for tobacco with the improved distillation device, and then absorbed by 5% (w) NaOH solution. H₂S was determined by ion chromatography with pulsed amperometric detection, IonPac AS7 anion exchange column as separation column, and the mixture of 1.5 mol·L^-1 NaOH-1 mol·L^-1 CH₃COONa-2% (φ) ethylenediamine (40+50+10) as the mobile phase. Linear relationship between values of peak area and mass concentration of H₂S was obtained in the range of 0.05-10 mg·L^-1, with detection limits (3S/N) of 3.2 μg·L^-1. Values of the spiked recovery were found in the range of 92.0%-95.4%, and RSDs (n=7) of intra-day and RSDs (n=7) of inter-day were all less than 4.0%.