LC-MS/MS Determination of 4 Food Borne Plant Toxins in Blood

LC-MS/MS was applied to the determination of 4 food borne plant toxins, i.e. coniine, cytisine, colchicine and solanine alkaloids in blood. Hemoglobin in 0.2 mL of blood sample was removed with 0.8 mL of acetonitrile. After centrifugation,the supernatant was filtered on 0.22 μm organic microporous filter membrane. Hypurity C₁₈ chromatographic column was used as stationary phase, and the mixture of 0.2% (φ) acetic acid solution (containing 5 mmol·L^-1 ammonium acetate, pH was 4.0) and methanol (20+80) was used as mobile phase in isometric elution. The full scanning mode and the selected reaction monitoring mode were adopted in MS/MS. Linear relationships between values of peak area and mass concentration of the 4 food borne plant toxins were kept in the same range of 5.0-500 μg·L^-1, with the same detection limit (3S/N) of 2.0 μg·L^-1 and the same lower limit of determination (10S/N) of 5.0 μg·L^-1. Tests for recovery were made by standard addition method at the concentration levels of 50, 200, 500 μg·L^-1, giving values of recovery and RSDs (n=5) in the ranges of 84.5%-111% and 1.5%-6.2% respectively.