Determination of 4 Aflatoxins in Cordyceps Taishanensis by High Performance Liquid Chromatography with QuEChERS Extraction

An aliquot (2.50 g) of the cordyceps taishanensis was taken and 7.5 mL of water and 10 mL of acetonitrile solution containing 0.1% (φ) acetic acid were added. The mixture was shaken vortically for 1 min, and then 4.0 g of anhydrous magnesium sulfate and 1.0 g of sodium chloride were added. After centrifugation, 1.0 mL of the supernatant was taken in the disperse solid phase extraction tube (2 mL, containing 150 mg of anhydrous magnesium sulfate, 50 mg N-propyl ethylenediamine, 50 mg of C₁₈ filler and 12 mg of graphitized carbon black). The mixture was shaken vortically for 30 s. The supernatant was filtered through 0.22 μm organic filter membrane, and the contents of 4 aflatoxins were determined by high performance liquid chromatography. As shown by the results, linear relationships between values of peak area and mass concentration of 4 aflatoxins were found in definite ranges, with detection limits (3S/N) in the range of 0.06-0.20 μg·kg^-1. Test for recovery was made by standard addition method, giving results in the range of 92.4%-102%, and RSDs (n=6) were found in the range of 2.7%-3.5%.