UHPLC Determination of 6 Coumarin Components in Angelica Dahurica from Different Localities

The powder of angelicae dahurica sample (about 2.5 g) was immersed in 25 mL of methanol overnight, and then extracted ultrasonically for 30 min. Appropriate amount of methanol was added to make up for lost mass. The extract was filtered on 0.22 μm organic membrane. UHPLC was applied to the determination of 6 coumarin components in the filtrate. Waters ACQUITY UPLC BEH-C₁₈ chromatographic column was used as separation column, and the mixture of acetonitrile and water mixed in different ratios was used as mobile phase in gradient elution. UV detector was adopted in the determination. Linear relationships between values of peak area and mass concentration of the 6 coumarin components were kept in definite ranges, with detection limits (3S/N) in the range of 0.034-0.091 mg·L^-1. The proposed method was applied to the analysis of angelicae dahurica sample, giving values of recovery found by standard addition method and RSDs (n=6) of determined values in the ranges of 93.2%-107% and 0.64%-1.7% respectively. Amounts of 6 coumarin components in 9 samples of angelica dahurica from different localities were processed by cluster analysis and principal component analysis. It was found that the results of cluster analysis and principal component analysis were consistent and the samples could be divided into 3 categories.