Simultaneous Determination of 3 Furfural Compounds in Expanded Cut Tobacco by UHPLC

The crushed sample (about 1 g) of expanded tobacco was extracted ultrasonically for 40 min with 20 mL of the mixture of acetonitrile and dichloromethane (1+1). Appropriate supernatant was centrifugated for 15 min at the speed of 10 000 r·min^-1 and filtered on 0.22 μm membrane. UHPLC was applied to the simultaneous determination of 3 furfural compounds in the filtrate. ACCQ-TAG^TMULTRA C₁₈ chromatographic column was used as separation column, and acetonitrile (1+9) solution was used as mobile phase. Detection at 284 nm was adopted in the determination. Linear relationships between values of peak area and mass concentration of the 3 furfural compounds were kept in definite ranges, with detection limits (3s) in the range of 0.012-0.015 mg·L^-1 and lower limits of determination (10s) in the range of 0.040-0.050 mg·L^-1. The proposed method was applied to the analysis of the sample of expanded cut tobacco, giving values of recovery found by standard addition method and RSDs (n=5) of determined values in the ranges of 76.1%-102% and 3.2%-4.9% respectively.