Determination of 7 Sweeteners in Cigarette Tip Paper by High Performance Liquid Chromatography with Evaporation Light Scattering Detection

2.000 0 g of the tip paper (cut to small pieces of 5 mm×5 mm) were extracted with 20 mL of H₂O by shaking with table for 35 min. The extract was taken and filtered through 0.22 μm filtering membrane. The filtrate was used for HPLC analysis with evaporation light scattering detection (ELSD). ZORBAX SB C₁₈ chromatographic column was used as stationary phase, and mixtures of (A) methanol (containing 0.1 mL of trifluoroacetic acid per liter, pH 4.5) and (B) water in various ratios were used as mobile phase in gradient elution. Volume of sample solution injected was 10 μL. Under this condition, 7 sweeteners were effectively separated. Standard curves of the 7 sweeteners were prepared by taking the values of peak areas of the sweeteners as abscissas, and the corresponding mass concentrations as ordinates, and the linearity ranges found were same between 70 to 400 mg·L^-1, with their detection limits (3s) in the range from 0.65 to 9.06 mg·L^-1. Test for recovery was made by addition of standards of the sweeteners at concentration levels to known samples as matrixes, giving values of average recovery in the range of 89.9% to 115%. RSDs (n=5) of the determined values were found in the range of 0.35% to 2.3%.