HPLC Determination of Residual Amounts of 19 Carbamate Pesticides in Vegetables

After homogenization, the vegetable sample was extracted with acetonitrile. SPE-NH₂ solid phase extraction column was used for purification. HPLC was applied to the determination of residual amounts of 19 carbamate pesticides in the purified liquid. PICKERING Carbamate Analysis-C₈ chromatographic column was used as separated column with mixtures of methanol and water with different mixing ratios as mobile phase in the gradient elution, and fluorescemce detector was used for determination after post column derivation. Excitation wavelength and emission wavelength of pirimicarb were set at 339, 392 nm, respectively. Excitation wavelength and emission wavelength of the other 18 carbamate pesticides were set at 339, 445 nm, respectively. Linear relationships between values of peak area and mass concentration of the 19 carbamate pesticides were kept in definite ranges, with detection limits (3S/N) in the range of 0.002-0.014 mg·kg^-1. On the base of blank cauliflower sample, test for recovery was made by standard addition method; values of recovery found were in the range of 47.7%-110%, with RSDs (n=6) of determined values in the range of 1.0%-12%.