HPLC-MS/MS Determination of Taxol and Cephalomannine in Liquor

The self-made Taxus fruit wine was injected into mass spectrometry, and the characteristic components of Taxus in liquor were determined as taxol and cephalomannine. The Taxus wine sample was diluted with 70% (φ) methanol solution. After centrifugation, HPLC-MS/MS was applied to the determination of taxol and cephalomannine in the supernatant. ACQUITY UPLC R BEH C₁₈ chromatographic column (2.1 mm×50 mm, 1.7 μm) was used as stationary phase, and the mixture of methanol and water mixed in different ratios was used as mobile phase in gradient elution. ESI⁺ and multi-reactions monitor mode were adopted in MS/MS. Linear relationships between values of peak area and mass concentration of taxol and cephalomannine were kept in definite ranges, with same detection limits (3S/N) of 2.5 μg·kg^-1 and same lower limits of determination (10S/N) of 8.3 μg·kg^-1. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 96.4%-116%, with RSDs (n=6) of determined values in the range of 1.1%-2.6%.

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