Systematic Analysis of Aroma Components in Salvia Sclarea by HS-SPME-GC-MS

HS-SPME-GC-MS was used to analyze the changes of aroma components from different parts (flower, stem, leaf and root) of Salvia sclarea planted in different plots (middle plot, south plot and north plot) during the flowering and harvest periods. The whole fresh sample of Salvia sclarea was dried naturally, and the root, flower, stem and leaf were separated. After crushing and sieving, the samples were placed in headspace bottles for HS-SPME treatment. The target compounds were separated on DB-5MS capillary column under temperature programmed conditions, and detected by MS equipped with electron impact ion source. Retention time and qualitative ions were used for qualitation, and the relative contents of aroma components were calculated by area normalization method. The results showed that 8, 17, 13 and 12 kinds of aroma components were found in the root, flower, stem and leaf of Salvia sclarea during the flowering period, and 17, 20, 16 and 24 were found during the harvest period. The main aroma components were alcohols, esters, alcohols and hydrocarbons (64.02%, 68.33%, 50.20% and 37.13%, average relative content, the same below) in the flowering period and all esters (81.46%, 77.80%, 84.05% and 68.27%) during the harvest period from the root, flower, stem and leaf of Salvia sclarea planted in different plots; linalool, myrcene and linalool, myrcene, and linalool were the common aroma components from root, flower, stem and leaf of Salvia Sclarea planted in different plots during different growth periods. Linalyl anthranilate was the peculiar substance of the root, flower and stem of Salvia sclarea planted in the southern plot in the flowering period, with a relative content more than 50%; the types of aroma components in the harvest period were higher than those in the flowering period, and the main aroma component was all linalyl acetate.