Determination of Acrylamide in Instant Coffee and Cookies by UHPLC-MS/MS

A method for the determination of acrylamide in instant coffee and cookies by UHPLC-MS/MS was developed. Instant coffee or cookies samples of 2.0 g was extracted twice with acetonitrile (10 mL×2) and degreased twice with hexane (10 mL×2). Acetonitrile phase of 10 mL was concentrated to nearly dryness under reducing pressure at 40 ℃. Water of 1 mL was added to the residue, and the solution obtained was filtered by 0.45 μm water filtration membrane. Aglient EclipsePlus C₁₈ column was used as stationary phase, and 5% (φ) methanol solution at a flow rate of 0.15 mL·min^-1 was used as mobile phase for isocratic elution. Acrylamide was detected by ESI⁺ and MRM mode, and quantified by internal standard method. As shown by the results, linear range of the standard curve of acrylamide was 0.005-1.00 mg·L^-1, with detection limit (3S/N) of 0.000 3 mg·L^-1. Recovery test was made on the instant coffee and cookies samples at 3 concentration levels by standard addition method, giving results in the range of 88.9%-111%, with RSDs (n=5) of the determined values ranged from 2.5% to 14%.