Determination of 9 Chemical Components of Coumarins in Angelica Dahurica by High Performance Liquid Chromatography and Its Multivariate Statistical Analysis

A method of high performance liquid chromatography (HPLC) was used to simultaneously determine 9 chemical components of coumarins in 30 batches of Angelica dahurica, inclding imperatorin, isoimperatorin, psoralen, oxypeucedaninhydrate, bergapten, byak-angelicol, xanthotoxol, oxypeucedanin and byak-angelicin. Then the index components of Angelica dahurica were screened by cluster analysis (CA), principal component analysis (PCA), and orthogonal partial least square-discriminant analysis (OPLS-DA). As shown by the results, linear relationships between values of peak area and mass concentration of 9 chemical components of coumarins were found in definite ranges, with detection limits (3S/N) ranged from 0.1 mg·L^-1 to 0.7 mg·L^-1. Test for recovery was made by standard addition method, giving results in the range of 99.1%-106%, with RSDs (n=6) of the determined values less than 3.0%. According to statistical analysis for the determined values of 9 chemical components of coumarins, it was shown that the quality of Angelica dahurica from different producing regions was different, and sulfur fumigation had a great influence on the quality of Angelica dahurica. The results of CA, PCA and OPLS-DA were consistent and the samples were divided into three categories. Imperatorin and oxypeucedanin were identified as the 2 index components with significant difference contribution.