Identification of 6 Prohibiting Illegally Added Drugs in Diet Food by Surface Enhanced Raman Spectrometry

A method for the identification of 6 prohibiting illegally added drugs in diet food, including sibutramine, hydrochlorothiazide, fluoxetine, phenolphthalein, furosemide, and N-benzoylsibutramine, was proposed by surface enhanced Raman spectrometry (SERS). Nanogold with the particle size of 50 nm was prepared as the surface strengthening agent, 30.00% (mass fraction) sodium chloride solution was used as the auxiliary agent, and SERS was used to identify 6 prohibiting illegally added drugs in diet food under the optimized orthogonal test conditions. As shown by the results, the detection limits of phenolphthalein, sibutramine, furosemide, fluoxetine, hydrochlorothiazide and N-benzoylsibutramine were 1.00, 20.0, 20.0, 35.0, 50.0, 50.0 mg·L^-1, respectively. Repeatability test for sibutramine, fluoxetine and phenolphthalein was carried out, with RSDs (n=8) of the characteristic peak signal intensity in the range of 2.8%-8.6%. This method was applied to rapidly screening 20 actual diet food samples, and the results were consistent with those of liquid chromatography-mass spectrometry in the laboratory.