Comparison of the Application of Four Spectra Analysis Techniques with Chemometrics in Production Area Differentiation of Sichuan Ophiopogon Japonicus

Hydrogen magnetic resonance spectroscopy (¹H-NMR), infrared spectroscopy (IR), ultraviolet spectroscopy (UV) and high performance liquid chromatography (HPLC) with chemometrics were used to distinguish the production area of Sichuan Ophiopogon japonicus, and the differentiation results of 4 spectral analysis techniques were compared. ¹H-NMR, IR, UV spectra and HPLC fingerprints of 11 batch of Sichuan Ophiopogon japonicus samples from different production areas were obtained, and the similarity evaluation was carried out. Cluster analysis, principal component analysis and partial least square-discriminant analysis were used to classify the samples. As shown by the results, the similarities of ¹H-NMR spectra and HPLC fingerprints of 8 batches of samples from Santai County, Mianyang City, Sichuan Province were greater than 0.950, showing a highly significant correlation (P<0.01), and that could be better distinguished from other production areas samples. The results of IR spectra with cluster analysis showed that 11 batches of samples could be divided into 3 categories, but those of UV, ¹H-NMR spectra and HPLC fingerprint could not subdivide the production areas. The results of ¹H-NMR, IR spectra and HPLC fingerprints with partial least square-discriminant analysis were consistent, that 8 batches of samples from Santai County, Mianyang City, Sichuan Province were distinguished from other production areas samples.