Quality Analysis of Artemisiae Argyi Folium by Ultra-High Performance Liquid Chromatography Fingerprint with Chemical Pattern Recognition

In order to provide reference for quality control of Artemisiae argyi Folium, the study was conducted by the title method mentioned. The fingerprints of 10 batches of Artemisiae argyi Folium samples from different producing regions were established by ultra-high performance liquid chromatography (UHPLC), and the common peaks were identified by comparison with the chromatogram of the mixed standard solution, and the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2012 A edition) was used for similarity evaluation. The method for the determination of isochlorogenic acid B, isochlorogenic acid C and jaceosidin by UHPLC were verified, and the 3 components in 10 batches of samples were quantitatively analyzed. The chemical pattern recognition of 10 batches of Artemisiae argyi Folium samples was carried out by cluster analysis and principal component analysis. It was shown that 17 common peaks were obtained from fingerprints of 10 batches of Artemisiae argyi Folium samples, among which 3 common peaks were identified, that were isochlorogenic acid B (peak 3), isochlorogenic acid C (peak 5) and jaceosidin (peak 8). Linear relationships between masses and peak areas of isochlorogenic acid B, isochlorogenic acid C and jaceosidin were kept in definite ranges, with detection limits (3S/N) in the range of 1.0×10^-5-8.0×10^-5ng. The results of methodology verification showed that the precision of instrument was good, and the method had good stability and repeatability. The mass fractions of isochlorogenic acid B, isochlorogenic acid C and jaceosidin in 10 batches of samples were in the ranges of 0.211 9-2.537 1 mg·g^-1, 0.165 8-3.365 0 mg·g^-1 and 0.169 1-0.931 8 mg·g^-1, respectively. The results of similarity evaluation showed that except S3 sample, the similarities of other samples were greater than 0.900. The results of cluster analysis and principal component analysis were consistent, and 10 batches of Artemisiae argyi Folium samples were divided into two categories. The S3 sample was classified into one category, and the other samples were one category.