Determination of 14 Phenolics Migration in Children Consumer Products by Liquid Chromatography-Tandem Mass Spectrometry with Solid Phase Extraction

When children came into contact with children consumer products through their hands and mouth, phenolics such as bisphenol A would migrate into the body through saliva or sweat, affecting children health. However, there were few reports on the detection of phenolics migration in saliva and sweat, so research mentioned by the title was conducted. The samples were cut or crushed into particles with diameter smaller than 2 mm, and an aliquot (1.00 g) was mixed with 20 mL of simulated sweat or simulated saliva for soaking for 2.0 h. An aliquot (10 mL) was passed through the Oasis HLB solid phase extraction column activated beforehand, then the column was rinsed with 5 mL of water, and eluted with 8 mL of methanol. The eluate was collected, blown to near dryness by nitrogen, diluted to 2.0 mL with 30% (volume fraction) methanol solution, and passed through a 0.22 μm filter membrane, and the filtrate was introduced into the liquid chromatograph-tandem mass spectrometer. Targets were separated on the Atlantis^TM T3 chromatographic column with mixed solutions composed of methanol-acetonitrile mixed solution at volume ratio of 1:1 and water at different volume ratios by gradient elution, ionized by the negative ion mode of the electric spray ion source, detected by the multiple reaction monitoring mode, and quantified by the matrix matching method. As found by the results, linear relationships between values of the mass concentration and the peak area of 14 phenolics were kept in the ranges of 0.1-20.0 μg·L^-1 (bisphenol A, bisphenol AF and tetrachlorobisphenol A) and 0.5-100.0 μ g·L^-1 (other 11 phenolics), with detection limits (3S/N) in the range of 0.3-1.5 μg·kg^-1. Test for recovery was made according to standard addition method, giving recoveries of 14 phenolics in the ranges of 82.5%-106% in simulated sweat and 80.5%-108% in simulated saliva, respectively. RSDs (n=5) of the determined values were in the ranges of 0.40%-9.1% and 0.30%-8.7%, respectively. The proposed method was applied to the analysis of 6 positive samples of bisphenol A and nonylphenol, with migration amounts of bisphenol A and nonylphenol in the ranges of 0.26-6.98 mg·kg^-1 and 0.01-0.12 mg·kg^-1, respectively.