Correlation Comparison of Toxic Equivalents of 17 Dioxins in Solid Foods of Animal Origin Based on Chemically Activated Luciferase Expression Gene Method and Standard Method

In order to improve the applicability of chemically activated luciferase expression gene method (CALUX) in screening for toxic equivalents of dioxins in solid foods of animal origin, and to make it interconvertible with the evaluation results of the standard method high resolution gas chromatography-high resolution mass spectrometry (HRGC-HRGC), the toxic equivalents of 7 kinds of 2,3,7,8-substituted polychlorinated dibenzo-dioxins (PCDDs) and 10 kinds of 2,3,7,8-substituted polychlorinated dibenzo-furans (PCDFs) in three kinds of typical solid foods of animal origin including meat, seafood and formula milk based on CALUX and HRGC-HRGC were evaluated, and the correlation between the determined results of two methods was analyzed, and the conversion relationship and suitable sample type were determined. It was shown that the toxic equivalent (TEQ) lower limits of 17 targets in the samples based on HRGC-HRMS ranged from 0.001 to 0.106 pg·g^-1, and the TEQ upper limits ranged from 0.004 to 0.242 pg·g^-1, that were much lower than the limits of 3.5 pg·g^-1 stipulated in European Union regulation EC 1881/2006. The toxic equivalent (BEQ) values of 17 targets in meat, seafood, and formula milk powder samples based on CALUX were in the ranges of 0.74-1.30, 0.31-0.63 pg·g^-1, and 0.043-0.074 pg·g^-1, respectively. The correlation between BEQ and TEQ values of meat and formula milk samples was poor, while the correlation between BEQ and TEQ upper limit values of seafood samples was good.