Study on Content Distribution of 51 Inorganic Elements in Mineral Medicine Gypsum Fibrosum from Different Origins by Inductively Coupled Plasma Mass Spectrometry/Inductively Coupled Plasma Atomic Emission Spectrometry

To systematically understand the material basis and influencing factors of Gypsum Fibrosum, the title method was used to simultaneously determine the contents of 51 inorganic elements (constant elements, trace elements, rare earth elements, heavy metals and harmful elements) in Gypsum Fibrosum. The 0.200 0 g of Gypsum Fibrosum powder was taken, and 5 mL of nitric acid was added. After sealing and mixing well, the mixture was digested by microwave at 220 ℃ for 30 min. The digestion solution was diluted to 50 mL by water (stock solution), and an aliquot (1 mL) was taken and diluted to 50 mL by water (dilution solution). Sample dilution solution and sample stock solution were used for the determination of the Ca, S elements and remaining 49 elements, respectively. S, P and Si elements were determined by inductively coupled plasma atomic emission spectrometry, and the remaining 48 elements were determined by inductively coupled plasma mass spectrometry with internal standard method. IBM SPSS Statistics 26.0 software was used to perform correlation analysis on the 16 elements with high content. Orthogonal partial least squares discriminant analysis (OPLS-DA) in the Simca software was used to identify elements that caused origin differences. The differences in associated elements in Gypsum Fibrosum and the relationship between Gypsum Fibrosum inorganic elements and drug efficacy and safety were discussed. As found by analytical results of 21 batches of samples, the Ca and S contents in Gypsum Fibrosum were the highest, consisting with the theoretical values, and showing no significant difference in different origins (p>0.05). The contents of heavy metals and harmful elements such as Pb, As, and Hg were relatively low, which might originate from Gypsum Fibrosum mining and processing processes. Contents of elements such as Mg, Na, Mn, Fe, Cu, and Ti that might have synergistic antipyretic pharmacological effects described in references could be basically ignored compared to the content obtained through other pathways in the body, which varied greatly in Gypsum Fibrosum from different origins, indicating these elements might not be the key elements affecting the antipyretic effect of Gypsum Fibrosum. As found by correlation analysis results, 39 pairs of elements were significantly positively correlated (p<0.01), and 18 pairs of elements were positively correlated (p<0.05), which might be associated or symbiotic elements in Gypsum Fibrosum mineralization or mining processes. As found by OPLS-DA analytical results, Cu and Si might be elements that caused differences of Gypsum Fibrosum between Inner Mongolia and Shandong and Shanxi, while Na and P might be elements that caused differences of Gypsum Fibrosum between Shanxi and Shandong.