Fluorospectrometric Study on the Interaction of the Condensate of o-Vanillin and Arginine Schiff Base with Trypsin and Its Analytical Application

UV-spectrophotometry and fluorospectrophotometry were applied to the study on the interaction of condensate of o-vanillin and arginine schiff base with trypsin.It was found that the fluorescence of trypsin was quenched due to its interaction with the condensate,and it was proved by the Stern-Volmer equation and Lineweaver-Burk equation that the fluorescence quenching was a static process.Equilibrium constant of combination (KA) and number of combination sites (n) were determined and the values obtained were 2.5×103L·mol-1 and 1 respectively.Linear relationship was found between values of ΔF(F0-F)determined at wavelengths of 292 nm (λex) and 339 nm (λem) and concentration of trypsin in the range of 0.016 7-0.150 6 g·L-1,and detection limit (3σ/k) of the method found was 5.02×10-3g·L-1.The proposed method was used in the determination of trypsin in simulated sample,giving values of recovery ranged from 94.9%-103.7%,and of RSD (n=7) of 1.92%.