Application of Capillary Electrophoretic Finger Print Atlas to the Determination of Flavones in the TCM of Phyllanthus Urinarial

The electrophoretic finger print atlas of flavones in the TCM of phyllanthus urinarial was established by capillary electrophoresis.A mixture of 60 mmol·L-1 borax solution and 16 mmol·L-1 sodium dodecyl sufate solution (adjusted to pH 9 with 0.1 mmol·L-1 boric acid solution preliminarily) was used as background electrolyte,and moving voltage of 15 kV was applied.UV-detection at the wavelength of 245 nm was adopted.Samples were introduced hydrostatically at a height of 12 cm for 15 s.Samples of phyllanthus urinarial grown in 10 different areas were taken for analysis.Base on the value of rate of common electrophoretic peaks fi≥70%,a set of electrophoretic finger print atlas of 8 peaks was determined for flavones in phyllanthus urinarial samples from 10 different areas in our country.Good similarities between the electrophoretic finger print atlases of the samples and the standards were obtained.Relative standard deviation among the values of relative migration times of various finger print peaks obtained at different time of sample introduction was less than 5%,and values of RSD′s for the relative peak areas were in the range from 3.0% to 7.8%.The results obtained proved that the sample was stable within 48 h.