Determination of Tetramine in Biological Samples by HPLC

A method of HPLC determination of tetramine in biological samples was reported in this paper.Tetramine in the sample was extracted with ethyl acetate and the extract was used for HPLC analysis.Separation was carried out on a C8 column (ZORBAX Eclipse XDB-C8,4.6 mm×150 mm,5 μm) with mobile phase of acetonitrile-water (15+85,V/V).UV-detection was made at the wavelength 220 nm.Linearity of the calibration curve was ranged in 5~500 μg·L-1 with a correlation coefficient of 0.999 9.Detection limit (S/N=3) was found to be 0.5 μg·kg-1.Recovery of the method was in range of 92.3%-98.5% and RSDs of the determination were in range of 2.67%-4.53%.The proposed method has been applied to the determination of tetramine in biological samples,including blood phasma,liver,heart and gastric contents.