Determination of Seven Organic Acids in Seabuckthorn Fruit Powder by IC

A method of ion chromatography was applied for the measurement of seven organic acids in the seabuckthorn fruit powder. The sample was reacted with KOH solution under oscillation,and then was filtrated with 0.22 μm filter membrane. The filtrate was separated on IonPac AG19 guard column and IonPac AS19 analytical column,and KOH solution was used for gradient elution. Conductivity detection was adopted. The linear relationships were found between the peak area and the mass concentration of the seven organic acids were in a certain range. Recovery rate measured by standard addition method was in the range of 94.0%-109%,with RSD (n=11) in the range of 0.20%-3.1%.