HPLC Determination of Main Oxidation Product of Mesitylene and Its Intermediate Products

HPLC was applied to the determinations of the main oxidation and intermediate products of mesitylene,including trimesic acid,mesitylenic acid and 5-methyl-1,3-benzenedicarboxylic acid.The conditions for separation and determination by HPLC were tested and optimized.The SHIMADZU C18 column (150 mm×4.6 mm,5 μm) was used as stationary phase,and a mixed solution of methanol,water and pH 5.0 acetate buffer solution (mixed in the a ratio of 79 to 20 to 1) was used as mobile phase.UV-detection at 225 nm was adopted in the determination.Linear relationships between the values of peak area and mass concentration of mesitylene,trimesic acid,mesitylenic acid and 5-methyl-1,3-benzenedicarboxylic acid in definite ranges were obtained.The values of recovery found by standard addition method were in ranges of 99.5%-100.4%,99.7%-100.8%,99.6%-100.8% and 99.3%-101.0% respectively.The values of RSD′s (n=6) were less than 2.0%.