A Modified FI-Differential Spectrophotometric Method for Determination of Total Saccharides in Pumpkin

Manifold of a FI-spectrophotometric installation for determination of total saccharides in pumpkin was designed and fabricated.Two sets of self-made flow-cells were installed separately in the light paths of sample and blank (or reference) solution.As shown in the figure,6 inflection points (A1,A2,A3,A4,A5 and A6) were observed in the differential absorption curve at one sample introduction.Based on the response signals at the inflection points,linear relationships between values of ΔAT and concentration of total saccharides was obtained in the range of 0.3-46 mg·L-1.Tests for precision and recovery were made on analysis of 3 samples of known contents of total saccharides and of addition of 11 mg·L-1 of standard saccharide solutions,values of RSD′s (n=5) found were ranged from 0.6% to 1.3%,and values of recovery in the range of 98.7%-101.2% were obtained.