UHPLC Determination of Residual Amounts of 3 Quinolone Drugs in Aquatic Products

A method of UHPLC for determination of residual amounts of 3 quinolone drugs, including norfloxacin, ciprofloxacin and enrofloxacin in aquatic products was proposed. The sample was extracted with acidified acetonitrile, and the extract was evaporated to dryness under reduced pressure. The residue obtained was dissolved in the mixed solvent of mobile phase, and defatted with n-hexane. The ACQUITY UPLCTM BEH C18 column was used as stationary phase, and a mixture of acetonitrile and formic acid solution (0.1+99.9) mixed in the ratio of 10 to 90 (by volume) was used as mobile phase. Fluorimetric detection at the wavelengths of (λex) 280 nm and (λem) 450 nm was adopted. Ranges of linearity found were same between 1.25-500 μg·L-1. Values of detection limit (3S/N) and lower limit of determination (10S/N) for 3 drugs were found same to be 0.1 μg·kg-1 and 0.3 μg·kg-1 respectively. Blank samples of pomfret, prawn and crab were analyzed by this method, and test for recovery was made on these matrixes by standard addition method, giving results of over 80%.