UHPLC-MS/MS Determination of Coumarin in Essence

The sample of essence was extracted with ethanol,and the solution obtained was determined rapidly by UPLC. The ACQUITYTM BEH C18 column was used as stationary phase;and a mixture of acetonitrile and acetic acid was used as mobile phase;positive electrospray ionization as well as multiple reaction monitoring mode was used in the detection. Ion pair used for qualitative analysis was: m/z 147.2,65.0,ion pair use for quantitative analysis was m/z 147.2,91.0. Linearity range was found between 20-8 000 μg·L-1,and the detection limit (3S/N) found was 10 μg·L-1. Values of recovery found by addition of standard coumarin were found in the range of 96.6%-101.0%,and values of RSD (n=5) was in the range of 3.9%-5.7%.