RP-HPLC Determination of Four Resveratrols in Grape Wine

RP-HPLC was applied to the simultaneous determinations of four resveratrols including trans-resveratrol,cis-resveratrol,trans-resveratrol glucoside and cis-resveratrol glucoside in grape wine.The chromatographic conditions of separation were tested and optimized.The four resveratrols were separated by gradient elution with mixed solutions of acetonitrile (φ 10%) and acetonitrile (φ 90%) with different ratios on GL Inertsil ODS-3 C18 column (4.6 mm×250 mm,5 μm),and their contents were determined by UV-detection at 306 nm.Linear relationships between the values of peak area and mass of four resveratrols were obtained.The values of recovery found by standard addition method were in range of 95.73%-102.15%,and values of RSD′s (n=6) were in the range of 3.12%-4.42%.