Determination of Saccharides in Food by Ion Exchange Chromatography with Integral Pulse Amperometric Detection

Ion exchange chromatography with integral pulse amperometric detection was applied to the determination of glucose,fructose,sucrose,lactose and maltose in food.Retention property of the 5 saccharides in the elution with NaOH solution was studied.CarboPacTM PA 10 column was used as separation column,and mixtures of pure water and 0.2 mol·L-1 NaOH solution in different ratio were used as mobile phase in the gradient elution.The flow rate taken was 0.5 mL·min-1.In the amperometric detection,gold electrode was used as working electrode,Ag/AgCl electrode was used as reference electrode.Under the optimum conditions,the 5 saccharides in a sample were separated and determined effectively and simultaneously.Linearity ranges for all the 5 saccharides were obtained in the range of 0.5-10.0 mg·L-1.Detection limit (3S/N) found were in the range of 0.8-2.5 μg·L-1.Test for recovery was made by addition of the standards of the 5 saccharides to a substantial sample as the matrix,values of recovery obtained were in the range of 91.1%-104.8%.