HPLC Determination of a New Herbicide (ZJ0777) and Its Hydrolysate

The Diamonisil (TM)-C18 column was used as stationary column and a mixture of methanol and H2O (mixed in the ratio of 60 to 40) was used as mobile phase with flow rate of 1.0 mL·min-1,in the HPLC determination of the new herbicide,ZJ0777,and its product of hydrolysis.Linear relationship between values of peak area and mass concentration of ZJ0777 was kept in the range from 5 to 100 mg·L-1.Values of RSD (n=8) found at the ZJ0777 concentration of 70.36 mg·L-1 by measuring of the peak areas was 3.8%.Recovery was tested by standard addition method,giving value of average recovery 100.9%.It was found that rapid hydrolysis of ZJ0777 was observed in alkaline medium and at higher temperatures and complete hydrolysis of ZJ0777 was achieved in alkaline medium of pH 12 at 25 ℃ after 1 day of reaction.