UHPLC-MS/MS Determination of Residual Amounts of 8 Estrogens in Meat Products

UHPLC-MS/MS was applied to the determination of residual amounts of 8 kinds of estrogens, including octylphenol, 4-nonylphenol, bishphenol A, diethylstilbestrol, estrone, 17β-estradiol, 17α-ethinylestradiol and estriol, in meat products. The sample was extracted twice with acetic ether, which was concentrated and purified by HLB solid-phase extraction column. The eluate was evaporated to near dryness by N2-blowing and the residue was dissolved with methanol-water (1+9). ACQUITYTM BEH C18 chromatographic column was used for separation, using the mixed solutions of 5 mmol·L-1 ammonium acetate (containing φ 0.1% formic acid) and methanol as mobile phase in the gradient elution. ESI with negative ionization mode and multi-reactions monitoring mode were used in the MS/MS analysis. Values of detection limits (3S/N) found were in the range of 0.2-0.3 μg·kg-1. Values of recovery and RSD′s (n=6) were found in the ranges of 76.2%-108.3% and 4.3%-11.7%, respectively.