RP-HPLC Determination of Tripterine in Phloem from Celastraceae Plants

RP-HPLC was applied to the determination of tripterine in phloem from celastraceae plants.The sample was treated by Soxhlet extraction with chloroform as solvent at 75 ℃.The extract obtained was separated on Kromasail C18 column (250 mm×4.6 mm,5 μm) with a mixture of acetonitrile and 0.005 mol·L-1 phosphoric acid solution (mixed in the ratio of 80 to 20) as mobile phase and the content of tripterine was determined spectrophotometrically at the wavelength of 425 nm.Linear relationship between the values of peak area and mass concentration of tripterine was obtained in the range of 0.021 32-0.106 6 g·L-1.The value of average recovery found by standard addition method was 97.2%.The proposed method was used in the analysis of 18 kinds of celastraceae plants,and the highest content (1.41%) of tripterine was found in celastraceae orbiculatus.