ICP-AES Determination of 9 Metal Elements in Biochemical Samples

Nine elements, i.e., Ca, Cu, Fe, K, Mg, Mn, Na, P and Zn, in biochemical samples were determined by ICP-AES. The sample was digested in a sealed PTFE vessel at 100 ℃ for 1 h with 1.0 mL of 250 g·L-1 tetramethylammonium hydroxide. The sample solution obtained was made up to 10 mL with HCl (10+90) solution. Values of detection limit (3s/k) of the method found were in the range of 0.003-0.11 mg·L-1. The proposed method was applied to the analysis of several CRM′s, the results obtained were found to be in consistency with the certified values.