RP-HPLC Determination of Chlorogenic Acid and Caffeic Acid in A Stomatitis Curing Sprays

One mL of the stomatitis curing sprays was taken and diluted with CH3OH-H2O(1+1) to 10 mL in a brown glass volumetric flask.An aliquot of 25 μL was taken for RP-HPLC determination of chlorogenic acid and caffeic acid.Shim-pack VP-ODS chromatographic column was used for separation and a mixed solution of methanol and 0.012 mol·L-1 H3PO4 (27+73) was used as mobile phase.Column temperature of 30 ℃,flow-rate of 0.8 mL·min-1 and UV-detection at 323 nm were adopted in the analysis.Linear relationships between values of peak area and mass concentration of chlorogenic acid and caffeic acid were obtained in the ranges of 0.51-91.80 mg·L-1 and 2.0-360.0 mg·L-1 respectively.Values of detection limit (3S/N) and lower limit of determination (10S/N) found were 0.10,0.40 mg·L-1 and 0.36,1.40 mg·L-1 respectively.Tests for recovery were made and the results obtained were in the ranges of 95.9%-97.4% (for chlorogenic acid) and 100.1%-100.9% (for caffeic acid).