HPLC Determination of Atrazine in Surface Water with SPE Packed with Molecularly Imprinted Polymer

The molecularly imprinted polymer (MIP′s) of atrazine was synthesized and packed into the SPE column which was used for separation and enrichment of atrazine from the water samples. Conditions for the MIP-SPE,including solvents used for sample introduction,for use as eluant and their amounts required,were studied and optimized. Water sample was introduced directly onto the MIP-SPE column,and rinsed with CH3OH-H2O(1+4). After drying the column by suction,atrazine on the column was eluted with methanol,which was collected and used for HPLC determination. Multiple of enrichment of atrazine by the proposed MIP-SPE attained to a value of 82. Linearity range for the determination was between 1 to 200 μg·L-1 and the detection limit (3S/N) found was 0.5 μg·L-1. Test for recovery was made at concentration levels of 10 μg·L-1 and 100 μg·L-1 of atrazine added as standard solution,values of recovery found were 96.0% RSD (n=6) 1.8% and 97.2% RSD (n=6) 3.1% respectively.