Determination of Curcumin in Curcuma by Capillary Electrophoresis with High Frequency Conductivity Detection in Nonaqueous Medium

It was reported in this paper that curcumine was separated from curcuma and determined by capillary electrophoresis with high-frequency conductivity detection in non-aqueous medium within 12 min. Optimum conditions for the analysis was described as follows: ① separation medium: anhydrous methanol;② electrolyte: 6 mmol·L-1 HOAc+8 mmol·L-1 NH4OAc solutions (pH 4.5);③ separation voltage: 25.0 kV. Linear relationship was kept between peak area and concentration of curcumine in the range from 3.0 to 140.0 mg per L of solution (r=0.997),with a detection limit of 0.5 mg·L-1. Samples of curcuma grown in Sichuan,Guangdong and Guangxi were collected and analyzed by the proposed method. Values of recovery test were in the range from 95.3%-100.9%.