UHPLC-MS/MS Determination of Residual Amount of Enostrobilurin in Food

UHPLC-MS/MS was applied to determination of residual amount of enostrobilurin in food .The sample was extracted with acetonitrile and purified by dispersive solid phase extraction with primary secondary amine, C18 and anhydrous MgSO4 as extractants. The eluate obtained were used for UHPLC-MS/MS determination, in which Eclipse Plus C18 column (50 mm×2.1 mm,1.8 μm)was used as stationary phase, and a mixture of acetonitrile and 5 mmol·L-1 ammonium acetate (75+25) was used as mobile phase; positive electrospray ionization was used in the detection. Linear relationship between values of peak area and mass concentration of enostrobilurin was kept in the range within 50 μg·L-1, with detection limit (3S/N) of 1 μg·kg-1. On the base of blank sample, test for recovery was made by standard addition method; values of average recovery found were in the range of 76.7%-89.6%, with RSD′s (n=10) in the range of 4.2%-11%.