Ion-chromatographic Determination of Anions in Tobacco

Six anions (i.e.,F-,Cl-,NO-2,HPO2-4,NO-3 and SO2-4) in tobacco sample were separated by the method of matrix solid phase dispersion and determined by ion chromatography (IC).0.1 g of the sample was mixed with 0.4 g of graphitized carbon black granules and ground in a mortar to make it a homogeneous dispersion.The dispersion was then filled into a solid phase extraction column.The SPE column was eluted with hot water to dissolve the anions from the sample in the dispersion.An aliquot of the eluate was used for IC determination.YSA8-8164A-8 Anion chromatographic column was used as stationary phase,and a mixed solution of Na2CO3 and NaHCO3 was used as mobile phase.Conductivity detection was adopted.The above mentioned anions were separately within 12 min.Values of D.L.(3S/N) were below 0.025 mg·L-1.Tests for recovery and precision were made,giving values of recovery ranged from 96.4%-103.6%,and of RSD′s (n=5) less than 2.1%.