Determination of Malachite Green in Fish by HPLC Coupled with Molecular Imprinting Solid-Phase Extraction

Molecularly imprinted polymer for malachite green (MG) was synthesized by bulk polymerization and used as the absorbent for the solid phase extraction (SPE) column. The fish sample was extracted with acetonitrile. The extract was purified on the molecularly imprinted SPE column and eluted with a mixture of methanol and acetic acid. The eluent was separated on Eclipse Plus C18 column using a mixture of acetonitrile and 50 mmol·L-1 of acetate buffer solution as mobile phase. The detection wavelength was 620 nm. The linear range of MG was 1.00-50.0 μg·L-1,with detection limit (3S/N) of 0.62 μg·kg-1. The recovery rates measured by adding standard samples to blank fish sample were in the range of 86.6%-95.4% with RSD (n=5) in the range of and 3.6%-6.8%.