RP-HPLC Determination of N-｛3-3-(Dimethylamino)-1-oxo-2-propenyl-phenyl｝ Acetamide and Its Interrelated Substances

Sample of N-｛3-3-(dimethylamino)-1-oxo-2-propenyl-phenyl｝ acetamide (abbreviated as NDAOPPAM) was dissolved and diluted to 100.0 mL with the mobile phase of mixture of methanol and H2O (38+62).Diamonsil C18 chromatographic column was used as stationary phase.Under the conditions described,NDAOPPAM was completed separated from 3-acetamidoacetophenenone (AAMAPN) which is one of the reactants in the synthesis of NDAOPPAM,and determined with ultraviolet detection at wavelength of 236 nm.Linear relationship between values of peak area and concentration of NDAOPPAM was obtained in the range of 40.4-161.6 mg·L-1.Detection limit (3S/N) found was 1 ng·L-1.Values of within-day and inter-day RSD′s (n=6) found for NDAOPPAM were 0.53% and 0.89% respectively.The un-reacted reactant,AAMAPN,remained in the product NDAOPPAM was determined under the same way.Three samples taken from different batches were analyzed and contents of AAMAPN found were 0.32%,0.43% and 0.49% respectively.