Simultaneous Determination of Hydrocortisone and Dyclonine Hydrochloride in Qingda Cream by HPLC

HPLC was used to simultaneously measure hydrocortisone and dyclonine hydrochloride in Qingda cream. The Agilent C18 column was used as stationary phase, and a mixture of 0.015 mol·L-1 ammonium acetate solution and methanol (42+58) as mobile phase at the flow rate of 0.6 mL·min-1, the column temperature was maintained at 25 ℃ and the detection wavelength was 254 nm. The linear ranges of hydrocortisone and dyclonine hydrochloride were between 0.12-0.60 μg, and the detection limits (3S/N) were 0.622 mg·L-1 and 0.606 mg·L-1 respectively. Values of recovery were in the range of 97.6%-100% for hydrocortisone, and 99.3%-99.7% for dyclonine hydrochloride, and values of RSD′s (n=6) were 1.8% and 0.94%, respectively.