HPLC Determination of Residual Sulfanilamides in Food of Animal Origin

Residual amounts of sulfanilamide drugs in the sample were extracted with acetonitrile,and purified by extraction with n-hexane and adsorption on chromatographic column packed with basic aluminum oxide.Sulfanilamides in the eluate (acetonitrile and water mixed in the ratio of 3+7) were separated and determined by HPLC.A C18 column (250 mm×4.6 mm) was used as stationary phase,and mixed solutions of acetonitrile (A) and mixture of acetonitrile+acetic acid+water (20+1+79) (B) in different ratio were used as mobile phase in the gradient elution.UV-detections at 270 nm were used in the determinations.Linear relationship between values of peak area and mass concentration of the drugs was kept in the range of 0.05-1.00 mg·L-1.Limit of determination of the method was found to be 0.04 mg·kg-1.On the base of a meat sample,test for recovery was made by addition of standard solutions of 3 drugs,and values of recovery found were in the range of 73.65%-93.45%.