Determination of the Banned Pigment SudanⅠ-Ⅳ Red and Para Red in food Stuffs by HPLC

An accurate and sensitive method for the determination of the banned pigments sudan red and para red in foodstuffs by HPLC was proposed.Sudan red and para red in foodstuffs were extracted in a ultrasonic washing machine with acetonitrile.ZORBAX SB-C18 cloumn (4.6 mm×150 mm,5 μm) was used as the stationary phase,and mixtures of dil.HOAc(1+999) and acetonitrile in different ratios were used as mobile phase in the gradient elution,at a flow-rate of 1 mL·min-1.Photometric deterctions were made at 485 nm.Recoveries of sudan red and para red were in the range of 90%-98% and RSD′s (n=6) found were less than 5%.Detection limits of the method was found to be 0.01 μg·g-1.Linear relationship was kept in the range of 0.2-2.0 mg·L-1.