Study on Sieving and Separation of Active Components of Chinese Angelica by Biochromatography

Molecular biochromatography (MBC) was applied to the study on sieving and separation of active components of Chinese angelica. The matrix of monolithic column was prepared with glycidylmethacrylate as functional monomer by in-situ copolymerization. After activating the functional group with diethylamine, bovine serum albumin (BSA) was absorbed onto the matrix of monolithic column and a MBC column with BSA as stationary phase was formed. The sample of Chinese angelica was extracted with methanol, and the extract was filtered on 0.45 μm filtering membrane, the filtrate was separated on the MBC column, with Tris-HCl buffer solution (containing 2% by volume of methanol as mobile phase. DAD was adopted in the determination. It was found that the mechanism of retention of the active components on BSA was the combining action of a variety of factors, including hydrophobic and electrostatic actions as well as the spatial structure. The components retained on BSA column were analyzed by GC-MS, and 8 components were identified, including the main volatile component of ligustilide.